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| Registros recuperados: 27 | |
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| Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián. |
| Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach... |
| Tipo: Journal article |
Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002 |
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| Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando. |
| The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,... |
| Tipo: Journal article |
Palavras-chave: Cloning; Genome; Social representations; Transgenic. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003 |
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| Pádua,Gilda Pizzolante de; Jesus,Adriana Madeira Santos; Fronza,Vanoli; Arantes,Neylson Eustáquio; Zito,Roberto Kazuhiko. |
| The difficulty on identifying, lack of segregation systems and absence of suitable standards for coexistence of non trangenic and transgenic soybean are contributing for contaminations that occur during productive system. The objective of this study was to evaluate the efficiency of two methods for detecting mixtures of seeds genetically modified (GM) into samples of non-GM soybean, in a way that seed lots can be assessed within the standards established by seed legislation. Two sizes of soybean samples (200 and 400 seeds), cv. BRSMG 810C (non-GM) and BRSMG 850GRR (GM), were assessed with four contamination levels (addition of GM seeds, for obtaining 0.0%, 0.5%, 1.0%, and 1.5% contamination), and two detection methods: immunoassay of lateral flux (ILF) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Glyphosate; Transgenic. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000400007 |
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| Moreira Silva,André Felipe; Giraldeli,Ana Ligia; Soares da Silva,Gustavo; da Silva Araújo,Lucas; Nogueira Lima,Robson Josadaque; Góes de Oliveira,Diogo Alexandre; Paiola Albrecht,Alfredo Junior; Victoria Filho,Ricardo. |
| Abstract It is believed that ALS inhibitor herbicides in combination with ACCase inhibitors can be used as an alternative to glyphosate in weed control in RR2/STS soybeans. In the present study, the efficacy of ALS inhibitor herbicides, in combination with haloxyfop, is evaluated in the control of weeds for post- emergence application (V4) of RR/STS soybeans. The experiment is carried out in the 2017/18 season in Piracicaba, Sao Paulo (SP), Brazil. The cultivar BMX Garra RR2/STS is used, and the treatments were composed by the application of ALS inhibitor herbicides in association with haloxyfop. The experimental design was a randomized block with four replications. Were evaluated control and dry mass of weeds, symptoms of injury in soybean plants and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ACCase inhibitors; ALS inhibitors; Glycine max; Herbicides mixture; Transgenic. |
| Ano: 2021 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122021000300292 |
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| Guerra E., Guillermo A.; Aguilar Valdes, Alfredo; Cabral Martell, Agustin; Alvarado Martinez, Luis Felipe; Alvarado Martinez, Tomas Everardo; Arras Vota, Ana Maria; Moreno Medina, Salomon. |
| For decades, food security has been a worldwide problem affecting the poorest in order to find a possible solution to this dilemma that not only continues, but constantly grows with the increase in population. Agriculture, as the main food supplier, has evolved and increased its productivity, and has expanded its agricultural frontiers, since previous decades, in its attempts to solve this problem. If predominant conventional agriculture (mainly dedicated to productivity) is complemented with substantial increase of transgenic, organic, agroecological, greenhouse and hydroponic crops, it will be more feasible to avoid the loss of forest, the damage and loss of soils and at the same time diminish food insecurity. Within this context, the agribusiness... |
| Tipo: Journal Article |
Palavras-chave: Food security; Transgenic; Organics; Agro ecological; Hydroponics; Greenhouse; Agribusiness manager.; Agribusiness. |
| Ano: 2011 |
URL: http://purl.umn.edu/99477 |
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| BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; NICOLAU, M.; VERNETTI JUNIOR, F. de J.; STECKLING, C.; ROVERSI, T.; RUBIN, S. de A. L.; GONÇALVES, J. A.; CASTRO, R. L. de; GABE, N. L.; OLIVEIRA, M. A. R. de; VICENTE, D.; MATZENBACHER, R.; GIASSON, N. F.; BAGATINI, N. P.; SILVA, A. E. L. da. |
| bitstream/item/128741/1/ID41911LVdocumentos102-p41-58.pdf |
| Tipo: Capítulo em livro técnico (INFOTECA-E) |
Palavras-chave: Soybean; Transgenic. |
| Ano: 2010 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/882340 |
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| Ribas,Alessandra Ferreira; Pereira,Luiz Filipe Protasio; Vieira,Luiz Gonzaga E.. |
| In the last 15 years, considerable advances were made in coffee genetic transformation. Different research groups in the world have been able to transform coffee with genes for insect resistance, decaffeinated coffee, herbicide resistance and control of fruit maturation. Although the majority of the research is still limited to laboratory and greenhouse studies, initial field tests with transformed coffee are beginning to appear in the literature. In this review we provide an update on the state of coffee genetic transformation, presenting technical aspects related to tissue culture systems, strategies for selection and transformation with particle bombardment, as well as the use of Agrobacterium tumefaciens. We also discuss the potential applications of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium; Coffea; Biolistic; Genetic engineering; Transgenic. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100007 |
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| Tillmann,Maria Ângela André; West,Shirlie. |
| Advances in genetic engineering permit the modification of plants to be tolerant to certain herbicides that are usually not selective. For practical and commercial purposes, it is important to be able to detect the presence or absence of these traits in genotypes. The objective of this research was to develop a procedure for identifying genetically modified soybean (Glycine max L. Merr.) with resistance to the herbicide glyphosate. Two studies were conducted based on germination test. In the first study, soybean seeds were pre-imbibed in paper towel with the herbicide solutions, then transferred to moist paper towel for the germination test. In the second study, seeds were placed directly in herbicide solutions in plastic cups and tested for germination... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenic; Germination; Detection; Glyphosate. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000300017 |
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| Registros recuperados: 27 | |
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